Helpful Tips For Working In A Biosafety Cabinet

Let me introduce you to a biosafety cabinet which is a vital tool for laboratories handling hazardous biological materials. Remember: Proper usage, maintenance, decontamination and disinfection process steps are essential to ensure safety, prevent contamination, and maintain compliance with regulatory standards.

What is a biosafety cabinet?

A biosafety cabinet (BSC) is an enclosed, ventilated workspace designed to protect laboratory personnel, the environment, and the samples being handled. It utilizes HEPA or ULPA filtration to remove airborne contaminants and is classified into different types based on protection levels:

• Class I: Protects personnel and the environment but does not protect samples.
• Class II: Provides protection for personnel, the environment, and your product.
• Class III: Offers the highest level of containment, is a "GloveBox" and used for highly infectious materials.

Preparing to Work in a Biosafety Cabinet

Before working inside a biosafety cabinet, ensure:

• The cabinet is certified and functioning correctly.
• Proper personal protective equipment (PPE), such as gloves and lab coats, are worn.
• All necessary materials are placed inside the cabinet to minimize movement.
• The workspace is organized side to side to prevent airflow disruption.
• The interior surfaces have been disinfected.

Best Practices for Safe Work Inside a Biosafety Cabinet

To maximize safety and efficiency while working inside a biosafety cabinet:

• Check the cabinet's Magnehelic gauge, Minihelic gauge, or airflow monitor to ensure proper function.
• Minimize movement to maintain steady airflow.
• Do not block airflow grilles which can disrupt airflow and introduce contamination.
• Work from side to side to prevent cross-contamination.
• Use slow and deliberate movements to avoid disrupting airflow.
• Limit rapid movements, door openings, or unnecessary disturbances near the cabinet.
• Perform work at least four inches inside the cabinet behind the sash to prevent exposure.

Common Mistakes for Biosafety Cabinet users

Avoid these common errors when using a biosafety cabinet:

• Blocking airflow grilles with equipment, paper, or hands.
• Using open flames, which can disrupt airflow and create safety hazards.
• Overloading the workspace and using the work area for storage, leading to poor air circulation.
• Failing to properly disinfect before and after use.
• Leaning into the cabinet, which can alter airflow dynamics and reduce protection.

Proper Disinfection and Shutdown Procedures

Remember, following disinfection process steps is crucial for maintaining a sterile workspace:

1. Remove all materials and dispose of waste properly.
2. Apply an appropriate disinfectant (e.g., IPA or alcohol-based solution) to all surfaces. Bleach may be used as long as it is entirely rinsed off.
3. Wipe down all interior surfaces, ensuring adequate contact time.
4. Allow the cabinet to dry completely before shutdown.
5. Turn off the BSC blower, close the sash, and ensure proper airflow monitoring.

Routine Maintenance and Certification

To maintain optimal performance and safety:

• Perform regular disinfection / decontamination as per laboratory protocols.
• Schedule professional certification at least annually.
• Follow the certifier's recommendation on replacing HEPA filters when needed.
• Check airflow and alarm functions routinely.
• Ensure proper user training on sterile technique, good laboratory practices, and maintenance.

Conclusion

Proper use and maintenance of a biosafety cabinet are essential for laboratory safety. Following best practices, avoiding common mistakes, and adhering to decontamination process steps will help ensure a clean and efficient workspace, lengthening the life of the equipment, and protecting both personnel and research integrity.