PHCbi - 152S3001 - On-Chip Sort LS5

PHCbi - 152S3001 - On-Chip Sort LS5

PHCbi - 152S3001 - On-Chip Sort LS5

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3 Lasers, FS, SS, FL (6 colors), laser wavelength 405nm, 488nm, 637nm.

Features

On-chip Sort, The world's first cell sorter using adisposable microfluidic chip, designed for BioPharma andFACS Core Facilities

 

The On-chip Sort is the world's first cell sorter using a disposable microfluidic chip. Utilizing the advanced microfluidic technology and micro-molding technology, we have developed the world's first microfluidic chip-based sorter. Cell sorting has never been easier than with our disposable microfluidic chip-based system. The product is designed to fit the demands of BioPharma and FACS Core facilities.

 

Needs for Research

 

  • Minimal Cell Damage
  • On board laser/colors
  • Cell purity
  • Application variety
  • No contamination risks

 

Benefit

 

  • No cross contamination
  • Easy to use for downstream applications without cell damage
  • Variety of Fluorescent usability
  • Sort away from wanted cells
  • Capable of sorting a variety of cells
  • Broad selection of sheath fluid
  • Take out - redundant
  • No need washing for fluidics system

 

Advantages of On-chip Sort

 

Damage & Maintenance-free

 

From cell damage to contamination, On-chip cell sorter solves the problems of conventional cell sorters altogether.

 

Utilizing the most advanced microfluidic technology and micro-molding technology, we have developed a unique "microfluidic chip-based sorting" for the first time in the world. Cell sorting has never been easier with the exchangeable chip with in-built fluidic system. On-chip innovation beyond a cell sorter, that is what we are here to offer.

 

Sorting Technology

 

Jet-in-air or Cuvette hybrid cell sorters have multiple processes that triggers sorter-induced cellular stress (SICS). On-chip Sort allows for much gentler sorting of cells compared to conventional sorters by the use of microfluidic chip.

Specifications

Overview

  • Nominal Height:
    13" (33.02 cm)
  • Nominal Width:
    24.4" (61.98 cm)
  • Nominal Length:
    15.4" (39.12 cm)

Optical System and Detection Sensitivity

  • Laser:
    Up to 3 lasers from 488 nm, 405 nm, 561 nm and 638 nm, customization available
  • Laser Class:
    Class 1 laser (IEC 60825-1 2014)
  • Measurement Parameters:
    Forward scatter light (FSC), side-scatter light (SSC), 6 PMT
  • Size Detection Sensitivity:
    FSC < 0.5μm, SSC < 1.0 μm
  • Fluorescence Sensitivity:
    < 200 MESF FITC
  • Data Analysis Capability:
    4 decades, 18 bit
  • Pulse Analysis:
    Height, Area, Width
  • Detection Wavelength:
    FL1 (445/20 nm), FL2 (543/22 nm), FL3 (607/36 nm), FL4 (676/37 nm), FL5 (716/40 nm), FL6 (775/46 nm)

Fluid Channel System - Analysis and Sorting

  • Fluid Channel System - Flow Cell:
    Disposable microfluidic chip
  • Fluid Channel System - Chip Material:
    COP
  • Fluid Channel System - Channel Size:
    80 μm x 80 μm, 150 μm x 150 μm
  • Fluid Channel System - Flow Rate:
    500 mm/sec
  • Fluid Channel System - Sheath Buffer:
    Any liquid can be used as long as COP is not dissolved
  • Fluid Channel System - Sample Fluid Volume:
    10~1000 μL
  • Fluid Channel System - Sheath Fluid Volume:
    1~9ml
  • Analysis and Sorting - Sorting Method:
    "Flow shift" method in the microfluidic system
  • Analysis and Sorting - Purity:
    > 95% (depends on concentration)
  • Analysis and Sorting - Yield:
    > 80% (depends on condition)
  • Analysis and Sorting - Cell Damage:
    No
  • Analysis and Sorting - Cross-Contamination Free:
    Yes (because of the disposable chip)
  • Analysis and Sorting - Aseptic Sorting:
    Yes
  • Analysis and Sorting - Pressure:
    0.3 - 3psi
  • Analysis and Sorting - Maximum Detection Speed:
    4,000 events/sec
  • Analysis and Sorting - Maximum Sorting Speed:
    1,000 targets/sec
  • Analysis and Sorting - Start-up:
    5 min
  • Analysis and Sorting - Shutdown:
    10 sec (no cleaning necessary)

Safety - Weight - Operating PC - Power Supply

  • Safety - Generation of Aerosol:
    No
  • Weight:
    45 kg
  • Operating PC - PC:
    Laptop PC
  • Operating PC - OS:
    Windows 10, 64 bit
  • Operating PC - Data Format:
    Own format and FCS3.0
  • Power Supply - Power Requirement:
    AC100-240V, 50/60Hz
  • Power Supply - Power Consumption:
    < 240 VA
  • Power Supply - Specification Summary:
    Laser 1, FS, SS, FL (5 colors)

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