DeNovix - DS-C - Spectrophotometer

DeNovix - DS-C - Spectrophotometer
DeNovix - DS-C - Spectrophotometer

DeNovix - DS-C - Spectrophotometer

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Compact cuvette spectrophotometer for nucleic acid quantification, OD 600 and kinetics applications. Full UV-Vis wavelength range of 190-840 nm.

Features

The DeNovix DS-C Spectrophotometer offers outstanding dynamic range, sensitivity, flexibility, and ease of use. The instrument is ready right out of the box—no PC setup or software installation is required. From GMP compliance facilities to teaching labs, the versatile DS-C makes absorbance measurements simple with application-specific EasyApps™ software.

 

  • Intuitive, Compact & Stand-Alone: Outstanding dynamic range, sensitivity, flexibility, and ease of use. Full spectrum UV-Vis: 190 – 840 nm.
  • Powerful EasyApps™ Software: Easy-to-use, intuitive software designed by life scientists, for life scientists.
  • Flexible Export & Connectivity: Wi-Fi, USB, Email, Ethernet and more. Data app automatically saves results.

 

Kinetics

 

  • Create simple kinetics methods measuring two absorbance wavelengths over a user-defined measurement frequency and period. EasyApps streamlines the process to create and save custom methods and enables cuvette temperature control between 37° and 45° C.

 

Cell Culture OD 600

 

The high-particulate nature of bacterial cell cultures benefit from a longer measurement pathlength. The particulates in these samples scatter light, and a 10mm pathlength provides a longer cross-section of the sample for the spectrophotometer’s light beam to travel through. This ensures the highest degree of accuracy and reproducibility when measuring these types of samples.

 

Maintenance-Free Design

 

  • No need for routine recalibration or scheduled maintenance procedures—the DS-C Spectrophotometer is calibrated for life!

 

Accepts a Large Variety of Cuvettes

 

  • The DS-C is compatible with standard quartz or plastic cuvettes with an industry-standard 8.5 mm z-height. Select from standard, semi-micro cuvettes and specialized microvolume cuvettes. Pathlengths from 0.125 to 10 mm can be selected in EasyApps, providing assay flexibility and extended dynamic range. Upper concentration limit is extended with shorter pathlengths.

 

Ideal for Teaching Environments

 

With its stand-alone functionality and intuitive user interface, the DS-C is the perfect spectrophotometer for classroom settings.

 

Wide Range of Applications

 

Nucleic Acid Quantification

  • Separate pre-configured apps for optimized workflow
  • Sample contaminant alerts
  • Pre-configured and user-defined factors for ssDNA ensures appropriate concentration calculations
  • 260/280 and 260/230 ratios displayed
  • 11 pre-configured dyes and fluorophores for Microarray
  • Option to add an unlimited number of new dyes for quick recall in Microarray

 

Protein Quantification

 

  • Pre-configured and user-defined protein types for A280 and Labeled Proteins
  • Pre-configured Peptide app for easy peptide analysis at A205/215
  • Quick addition of new user-defined protein types for improved workflow
  • Ultra short pathlength enables high absorbance capability: up to 750 mg/mL BSA
  • 11 pre-configured dyes and fluorophores for Labeled Proteins
  • Option to add an unlimited number of new dyes for quick recall in Labeled Proteins
  • BCA, Bradford, Lowry, and 660 nm colorimetric assays optimized for ease of use
  • Quick save and recall of standard curves available

 

UV-Vis and Custom Apps

 

  • Simple but powerful apps enable measurement of more than just nucleic acids and proteins
  • Full spectrum analysis (190 – 840 nm)
  • Broad absorbance range for highly concentrated samples (up to 750 A, 1 cm equivalent)
  • Add new user-defined standard curve methods
  • Add custom formulas to eliminate downstream calculations

 

Fluorescence Quantification

 

  • Measurement ranges
    • 0.0005 ng/µL – 4000 ng/µL dsDNA
    • 0.25 – 1500 ng/µL RNA
  • Four user-selectable LED light sources – compatible with all commonly used quantification assays e.g. DeNovix assays, Thermo Fisher Qubit™ assays, Thermo Fisher Quant-iT™ assays, and Promega Quantifluor™ assays
  • Easy configuration of additional assays
  • Single or multi-replicate standard curve options
  • User control of line fit, trend line analysis, subtraction of blank
  • User control of assay and reported sample concentration units
  • Save and re-use curves and lists of standard concentrations
  • Using DeNovix Fluorescence Quantification assays. For other kits please see manufacturer’s specifications.

 

Custom Fluorescence Apps

 

  • Add new standard curve based fluorescent assays (nucleic assay, protein or other)
  • Basic Fluorometer app returns RFU values for a wide range of fluorophores

 

Kinetics and OD600

 

  • Full spectrum analysis (190 – 840 nm), with dual or single wavelength monitoring
  • Built-in heater with temperature control between 37° and 45° C.
  • Dual graphs enable absorbance vs. time or absorbance vs. wavelength plots
  • All kinetic data saved
  • Estimated cell number calculator included in OD 600 app

Specifications

Overview

  • Nominal Width:
    20" (50.8 cm)
  • Nominal Length:
    27" (68.58 cm)

General - Measurement Modes

  • Wi-Fi/Ethernet:
    Yes
  • Connectivity:
    Wi-Fi, Ethernet, Email, 2 USB ports
  • Export Formats:
    csv, pdf, jpg, network/label printers
  • Display:
    7” HD Color Touchscreen
  • Operating System:
    Custom Linux® OS
  • CPU:
    Quad-core Cortex-A72
  • User Input:
    Touch screen with swipe and pinch motion, glove compatible
  • Internal Storage:
    32GB flash drive
  • IQOQ Available:
    Yes
  • 21 CFR Pt 11 Ready:
    Yes
  • Contamination Detection:
    Purity Ratios with Contamination Alerts
  • AutoRun:
    No
  • Colors:
    Fire Red
  • Footprint:
    20 cm X 27 cm
  • Weight:
    1.5 kg
  • Operating Voltage:
    12 VDC
  • Operating Power Consumption:
    10 W, max 36 W
  • Approvals:
    CE, UL/CSA, FCC and Japan CAB
  • Manufacture Location:
    USA
  • Warranty:
    2 years
  • Patents:
    denovix.com/patents/
  • 1 µL UV-Vis Samples:
    None
  • Cuvette Absorbance:
    Yes
  • Fluorescence:
    Add On
  • Calibrated for Life:
    Yes

Absorbance Specifications

  • Measurement Speed:
    2 seconds
  • Light Source:
    Pulsed Xenon Flash Lamp
  • Wavelength Range:
    190 - 840 nm
  • Wavelength Accuracy:
    0.5 nm
  • Wavelength Precision:
    0.14 nm
  • Spectral Resolution:
    1.5 nm (FWHM at Hg 253.7 nm)
  • Absorbance Range:
    0.0008 - 1.5 AU (1 cm)
  • Absorbance Precision:
    0.015 AU (1 cm) or 1%, whichever is greater
  • Absorbance Accuracy:
    1.5% at 0.75 AU at 260 nm
  • Detector Type:
    2048 Element CCD

Cuvette UV-Vis Absorbance - Fluorescence

  • Detection Limit:
    0.04 ng/µL (dsDNA - 1 cm), 0.002 mg/mL (BSA - 1 cm)
  • Maximum Concentration:
    75 ng/µL (dsDNA - 1 cm), 2.25 mg/mL (BSA - 1 cm)
  • Beam Height:
    8.5 mm
  • Heating:
    37 - 45 °C +/- 0.5 °C
  • Pathlength:
    10, 5, 2, 1, 0.5, 0.2, 0.125 mm
  • Cuvette Dimensions:
    12.5 mm x 12.5 mm x 45 mm (LxWxH)
  • Absorbance Range:
    0.0008 - 1.5 AU (1 cm)
  • Kinetics:
    Yes
  • Fluorescence:
    Optional Fx Module
  • Light Sources:
    UV LED (~375 nm), Blue LED (~470 nm), Green LED (~525 nm), Red LED (~635 nm)
  • Excitation Filters:
    UV
  • Emission Filters:
    435 - 485 nm, 514 - 567 nm, 565 - 650 nm, 665 - 740 nm
  • Detection Limit (dsDNA):
    0.5 pg/µL
  • Maximum Concentration (dsDNA):
    4000 ng/µL
  • Detectors:
    Photodiode, Detection Range 300 - 1000 nm
  • Tube Type:
    0.5 mL Real Time thin-wall PCR tube (Polypropylene)
  • Measurement Speed:
    <2 seconds

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