DeNovix takes spectrophotometry to a whole new level of performance with the DS-11 Series. This easy-to-use, compact spectrophotometer delivers full spectrum UV-Vis analysis and fluorescence capability, making it ideal for rapid nucleic acid and protein quantification.

• Three Modes in One: Nano UV-Vis, Fluorescence and Cuvette options. Multi-modal spectrophotometer fluorometer for unmatched specificity and sensitivity.
• Powerful EasyApps™ Software: Easy-to-use, intuitive software designed by life scientists, for life scientists.
• SmartPath™ Technology: Designed for sensitivity and accuracy, SmartPath technology algorithms compress the sample so there’s never column breakage.
• Multi-Award Winning: Twice-awarded Life Science Product of the Year, Platinum Seal of Quality.
• Flexible Export & Connectivity: Wi-Fi, USB, Email, Ethernet and more. Data app automatically saves results.
• Maintenance-Free Design: No recalibration required for the life of the instrument.

Rapid and Reliable 1µL UV-Vis

SmartPath™ Technology

The DeNovix team developed a combination of opto-mechanical components and software algorithms that optimize results for every sample. Bridge Testing™ verification eliminates unreliable results that stem from broken sample columns or loading issues.

• Quantify DNA, RNA and protein in less than 3 seconds
• Widest dynamic range and lowest detection limit 0.75 – 37,500 ng/µL dsDNA
• No routine maintenance or recalibration required
• Full spectrum UV-Vis analysis using 0.5 – 1.0 µL of sample

SmartPath algorithms ensure that the sample is only compressed, never stretched, to keep the column intact.

SmartQC™ Contamination Alerts

Multiple algorithms ensure confidence in results while also providing important information about possible sample anomalies.

Integrated Fluorescence

Unmatched Sensitivity

The DS-11 Series uniquely integrates an optional 4-channel fluorometer. Fluorescence mode enables up to 1000x more sensitivity. DeNovix Fluorometers are compatible with a wide range of commonly used quantification assays.

• Full range of dsDNA and RNA assay kits
• Simple mix-and-measure, 2-point standard protocol
• Outperforms equivalent Thermo Fisher Qubit™ assay kits in both sensitivity and dynamic range
• Sensitivity: Extend the lower detection limit for low concentration samples. Quantify down to 0.5 pg/µL dsDNA
• Specificity: Selectively quantify a sample in the presence of contaminants using DeNovix Fluorescence Quantification Assays.
• Total Sample QC: Combine Absorbance and Fluorescence measurements for specific quantification and reliable contamination detection.

Cuvette Absorbance

Ultimate Flexibility

• Use standard quartz or disposable cuvettes for up to 10 mm pathlength absorbance measurements (190 – 840 nm).
• Cuvette applications include nucleic acids, proteins, colorimetric assays, OD600 and custom standard curves. Systems include a cuvette block heater for Kinetics studies from 37 – 45°C.

Wide Range of Applications

The DS-11 Series combines the ease of use, wide dynamic range, and contamination detection of UV-Vis with the enhanced sensitivity and specificity of fluorescence quantification. This unmatched flexibility allows the DS-11 Series to excel in a wide range of applications.

Nucleic Acid Quantification

• Measurement ranges
  • 0.75 – 37,500 ng/µL dsDNA
  • 0.6 – 30,000 ng/µL RNA
• Separate pre-configured apps for optimized workflow
• Sample contaminant alerts
• Pre-configured and user-defined factors for ssDNA ensures appropriate concentration calculations
• 260/280 and 260/230 ratios displayed
• 11 pre-configured dyes and fluorophores for Microarray
• Option to add an unlimited number of new dyes for quick recall in Microarray

Protein Quantification

• Measurement range
  • 0.04 – 1,125 mg/mL BSA
• Pre-configured and user-defined protein types for A280 and Labeled Proteins
• Pre-configured Peptide app for easy peptide analysis at A205/215
• Quick addition of new user defined protein types for improved workflow
• Ultra short pathlength enables high absorbance capability: up to 750 mg/mL BSA
• 11 pre-configured dyes and fluorophores for Labeled Proteins
• Option to add an unlimited number of new dyes for quick recall in Labeled Proteins
• BCA, Bradford, Lowry and 660 nm colorimetric assays optimized for ease of use
• Quick save and recall of standard curves available

Fluorescence Quantification

• Measurement ranges
  • 0.0005 ng/µL – 4000 ng/µL dsDNA
  • 0.25 – 1500 ng/µL RNA
• Four user-selectable LED light sources – compatible with all commonly used quantification assays e.g. DeNovix assays, Thermo Fisher Qubit™ assays, Thermo Fisher Quant-iT™ assays, and Promega Quantifluor™ assays
• Easy configuration of additional assays
• Single or multi-replicate standard curve options
• User control of line fit, trend line analysis, subtraction of blank
• User control of assay and reported sample concentration units
• Save and re-use curves and lists of standard concentrations
• Using DeNovix Fluorescence Quantification assays. For other kits please see manufacturer’s specifications.

Custom Fluorescence Apps

• Add new standard curve based fluorescent assays (nucleic assay, protein or other)
• Basic Fluorometer app returns RFU values for a wide range of fluorophores

Fluorescence Freedom!

DeNovix fluorometers give scientists the flexibility to choose any fluorescent assay, not just the assays supplied by one manufacturer. Fluorometer apps are compatible with DeNovix Fluorescence Assays and other common commercially available assays measured in standard 0.5 mL thin-wall PCR tubes.

Compatible Assays Include:

• dsDNA: DeNovix dsDNA Broad Range, DeNovix dsDNA High Sensitivity, DeNovix dsDNA Ultra High Sensitivity, Quant-iT PicoGreen™, Quant-iT dsDNA HS, Quant-iT dsDNA BR, Qubit™ dsDNA BR, Qubit™ dsDNA HS, Quantifluor™ dsDNA, Quantifluor™ One dsDNA, Qubit™ 1X dsDNA HS, Qubit™ 1X dsDNA BR
• RNA: DeNovix RNA Assay, Quant-iT Ribogreen, Quant-iT RNA BR, Quant-iT RNA HS, Qubit™ RNA BR, Qubit™ RNA HS, Quantifluor RNA, Quant-iT microRNA, Qubit™ MicroRNA
• ssDNA: Quant-iT OliGreen, Qubit™ ssDNA, Quantifluor ssDNA
• Protein: Quant-iT Protein, Qubit™ Protein, Qubit™ Protein BR

UV-Vis and Custom Apps

• Simple but powerful apps enable measurement of more than just nucleic acids and proteins
• Full spectrum analysis (190 – 840 nm)
• Broad absorbance range for highly concentrated samples (up to 750 A, 1 cm equivalent)
• Add new user-defined standard curve methods
• Add custom formulas to eliminate downstream calculations
• Add kinetics methods (DS-11+/DS-11 FX+ models)

Kinetics and OD600

• DS-11 FX+ and DS-11+ models enable cuvette-based kinetics studies
• Full spectrum analysis (190 – 840 nm), with dual or single wavelength monitoring
• Built-in heater with temperature control between 37° and 45° C.
• Dual graphs enable absorbance vs. time or absorbance vs. wavelength plots
• All kinetic data saved
• Estimated cell number calculator included in OD 600 app