LiCellMo Live Cell Metabolic Analyzer

Continuous, sampling-free measurement of glucose and lactate in culture medium. Visualize real-time changes in cell metabolism.

Cells are constantly growing and differentiating, and these processes are closely linked to metabolism. In the field of cancer immunology, stem cell research, and the development of manufacturing processes for cell-based formulations, understanding the metabolic state of cells is increasingly important for analyzing cell activation and disease. Continuous analysis of cell metabolism offers the ability to visualize the state of cells in real-time, creating opportunities for unprecedented discoveries in cell metabolism. The live cell metabolic analyzer, LiCellMo, helps open new doors to these discoveries.

In-line Sensors: The key to continuous measurements of glucose and lactate.

The glycolytic pathway is one of the main components of cellular energy metabolism. During glycolysis, glucose is taken up into cells and lactate is produced. Conventional analysis of cell metabolism typically involves estimating glucose and lactate concentrations from periodic sampling. The high-precision in-line sensors of LiCellMo offer unique advantages:

• Real-time monitoring of glucose and lactate concentrations.
• Continuous measurements.
• No sampling of the culture medium and use of the same cells for separate evaluation after measurement.
• Measure cells in their usual culture environment.

The simplicity of LiCellMo's design makes it suitable for any laboratory space and does not require specialized cell culturing equipment. Common commercial products (culture medium, 24-well plates, calibration liquid, additive reagents) can be used for cell culture. The sensor module and plate adaptor can be attached to standard 24-well plates. Once the plate is placed in the detector pre-installed in the CO2 incubator, real-time measurements can be easily monitored using the touch-panel controller. Optional plate adapters are available for five different commercial 24-well plates.

Evaluate Glycolytic Changes Directly

Changes in the glycolytic pathway can be evaluated directly by measuring the culture medium concentrations of glucose taken up by cells and lactate produced. With the PHCbi Live cell metabolic analyzer, the state of cell metabolism can be visualized as the rate of metabolic change using the consumption rate and production rate based on concentration values. Monitoring the efficiency of conversion from glucose to lactate makes it possible to evaluate not only glycolysis, but also changes in the balance with other cellular metabolic processes, such as oxidative phosphorylation.

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